Identification and Validation of Expressed Sequence Tags from Pigeonpea (Cajanus cajan L.) Root

Table 1

Primer sequences of subset of ESTs for RT-PCR analysis.

Accession number	Putative function	Optimum 𝑇𝑚 (°C)	Primer sequence (5′-3′)
JK973671	S-Adenosylmethionine synthetase	61	F-AGAGGAAAT CGGT GCTGGTG
			R-GCAGCAATTTGGTCGTTGGT
JK973674	Phosphoglycerate kinase	59	F-TCCCGATCCCGATACCCTAC
			R-CAGCACGCTTTTCAGCAGTT
JK973715	Serine carboxypeptidase	60	F-ACATGAAGCTCAGTGGAGGAG
			R-AGCCATGGCCTCCAATCTTC
JK973726	Methionine aminopeptidase	60	F-GGCAT TGAAAGTTGGGCAGG
			R-GATTGCAGCACCGACATCAC