Table of Contents
International Journal of Plant Genomics
Volume 2017 (2017), Article ID 5636314, 8 pages
https://doi.org/10.1155/2017/5636314
Research Article

Molecular Identification and Karyological Analysis of a Rampant Aspen Populus tremula L. (Salicaceae) Clone

1Laboratory of Population Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow 119991, Russia
2Laboratory of Biotechnologies, Sabinsky Training Experimental Forestry, Leskhoz, Republic of Tatarstan 422062, Russia
3Forest Genetics Department, Russian Center for Forest Health, Pushkino 141207, Russia
4Forest Biotechnology Group, Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch, Pushchino 142290, Russia

Correspondence should be addressed to Dmitry V. Politov; moc.liamg@71p.irtimd

Received 18 November 2016; Revised 17 February 2017; Accepted 26 February 2017; Published 23 April 2017

Academic Editor: Pierre Sourdille

Copyright © 2017 Dmitry V. Politov et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A rampant highly heterozygous aspen (Populus tremula L.) clone “Meshabash” has been revealed in course of population genetic diversity analysis in a native stand in the Republic of Tatarstan, Russia. Here we report the results of karyological analysis showing that this highly vigorous clone is diploid () while typically triploid aspen demonstrates increased growth rate and resistance to aspen trunk rot caused by fungus Phellinus tremulae. By means of DNA identification of a series of model trees using 14 SSR loci we outlined the area occupied by this clone (at least 1.94 ha) and demonstrated that its ramets constitute 40 out of 48 genotyped trunks on the plot with the maximal distance between ramets 254 m. Since aspen is able to regenerate after cutting or die-off of maternal tree by root suckers at a distance up to 20–35 m this assumed that current stand appeared as a result of such spreading from an ortet tree during at least 5 generations. Trunk rot damage in the wood of model trees indicated low influence of this pathogen on viability and performance of the studied clone that can be associated with its extreme heterozygosity level (0.926) exceeding all the studied trees in this research plot and in three other control samples.