Table 1: A summary of mutant sources of SNP markers and KASP genotyping assays developed and validated in various genetic materials for different seed composition traits used in the present study.

TraitMarkerAssay IDGenePositionEffect on traitDonor checksReference

Oleic acidFAD2-1A_17DMU-HO-1Glyma10g42470S117NImproves oil shelf life17 D[46]
FAD2-1A_A_DelMU-HO-2PI603452[47]
FAD2-1B_327MU-HO-3Glyma20G24530P137RPI283327[32]
FAD2-1B_I143TMU-HO-4I143TPI578451[32]

Linolenic acidFAD3A_DelMU-LL-1Glyma14g373506.4 kb del.Oxidative and flavor instabilityIA3024[31]
FAD3A_SpliceMU-LL-2G810ACX1512-44; SB-01[48]
FAD3B_SpliceMU-LL-3Glyma02g39230G462AIA3024[49]
FAD3C_G128EMU-LL-4Glyma18g06950G128ECX1512-44; SB-01[48]
FAD3C_H304YMU-LL-5H304YA29, IA3024[49]

Kunitz trypsin inhibitorKTIMU-KTI-1Glyma08g45530Q120AntinutritionalPI 542044[9]

Stachyose synthaseSTSMU-STS-1Glyma19g4055033 bp del.AntinutritionalSP6A-209[17]

Raffinose synthaseRS2_WMU-RS2-1Glyma06g18890TGG991AntinutritionalPI 200508[50]
RS2_397MU-RS2-2T107IAntinutritional397[46]

LipoxygenaseLox-1MU-Lox-1Glyma13g4232074 bp del.Beany flavorPI 408251[7]
Lox-2MU-Lox-2Glyma13g42310H532QBeany flavorJinpumkong-2[8]
Lox3_G_100MU-Lox-3Glyma15g03030G100Beany flavorPI 205085;
PI 417458
[7]

: deletion.