Figure 5: Validation of differential expression patterns for selected DEGs by qRT-PCR. Validation of the expression patterns for selected DEGs using qRT-PCR. Gene reference numbers were shown on the -axis; blue and red bars show the relative expression level in the roots of B.9 and G.935 from qRT-PCR experiment; the green pyramid indicates the values of Log2FC from RNA-seq data; the upward direction represents the higher transcript level in the roots of G.935 and downward direction indicates lower transcript level in G.935. Values on the axis denote the relative expression level for qRT-PCR and the Log2FC values from RNA-seq data set.