Wet Laboratory Tools Widely Used in Plant Genomics
1Biological Sciences and Bioengineering Program, Sabanci University, 34956 Istanbul, Turkey
2Laboratory for Plant Genomics and Molecular Genetics and the Texas A&M University GENEfinder Genomic Resources Center, Texas A&M University, TX 77843, USA
3Molecular Biology Laboratory, CCS University, Meerut, Uttar Pradesh 250005, India
4Unité de Recherche en Génomique Végétale, INRA-CNRS-UEVE, 2 rue Gaston Crémieux, 91057 Evry Cedex, France
5Centro de Investigación Científica de Yucatán, Loma Bonita, Col. Chuburná de Hidalgo, CP. 97200 Mérida, Yucatán, Mexico
6CSIRO Plant Industry, Queensland Bioscience Precinct, Bag 10,Clayton South VIC 3169, Australia
Wet Laboratory Tools Widely Used in Plant Genomics
Description
There are numerous laboratory tools developed and available for use in plant genomics; however, they are usually published in books or in journal separate issues, making them accessed and updated inconveniently. Collection/integration of the wet laboratory tools that are widely used, further improved and advanced in a special issue that is open-accessed, is crucial to and greatly helpful for research of plant genomics and other disciplines of plant biology. The tools below listed themes are called for this special issue, but other tools that are useful for plant genomics are always welcome. The reviewed articles in other systems (e.g., mammalian system) that are potentially applicable to plant genomics are also considered for publication in the special issue. The emphasis and themes are as follows:
- Cytogenetics Tools
- Fish, fiber-fish, and gish
- Chromosome banding methods (i.e., C-banding, N-banding) and karyotyping
- Microscopic analysis
- Molecular Tools
- Isolation of conventional and megabase-sized DNA and RNA isolation (including sRNAs)
- Construction of genomic DNA libraries (small-insert and shotgun libraries, large-insert BAC and BIBAC libraries) and cDNA libraries
- Screening large-insert libraries and targeting specific chromosome regions' tools (i.e., pools for PCR, filters)
- DNA sequencing (plasmid and BAC end sequencing, high-throughput 454 sequencing)
- Genetic and physical mapping, QTL analysis, and DNA markers
- Gene expression analyses (i.e., microarray analysis, qRT-PCR, SAGE, RAGE)
- Mutagenesis (physical and chemical mutagenesis, T-DNA tagging, transposable element)
- Virus-induced gene silencing and RNAi silencing and yeast two-hybrid system
- Methods for genetically modified organism analysis
- PCR methods (tail PCR, emulsion PCR, RACE-PCR, multiplex PCR, etc.)
- Analytical and Biochemical Tools
- SDS-PAGE and PAGE and two-dimensional gel electrophoresis
- Heterologous gene expression, production, and purification of recombinant proteins
- Immunoblotting and immunohistochemistry of proteins and DNA molecules
Authors should follow the International Journal of Plant Genomics manuscript format described at the journal site http://www.hindawi.com/journals/ijpg/. Prospective authors should submit an electronic copy of their complete manuscript through the journal Manuscript Tracking System at http://mts.hindawi.com/, according to the following timetable: