Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals
Figure 6
Comparison of results from toxicoproteomics experiments with Rotenone, DNOC, and Diclofenac exposure. (a) PCA scores when all samples are included in PCA analysis. (b) Venn-Diagramm based on results from substance-specific univariate spot-to-spot analysis. Most of the protein spots changed specifically. 9 proteins concurrently changed after Rotenone, DNOC, and Diclofenac treatment. Moreover, 13 proteins simultaneously changed after Rotenone and DNOC treatment, and 14 proteins showed changed expression levels at exposure against Diclofenac or Rotenone. Only one protein was found to be changed in the larval protein samples after DNOC as well as Diclofenac treatment. (c) 2-DE gel from eleutheroembryonal protein samples. All proteins that significantly changed in expression in at least two toxicoproteomics experiments (treatment with either Rotenone, DNOC, or Diclofenac) are labelled: Protein labels of protein spots simultaneously altered in Rotenone, DNOC, and Diclofenac experiments are framed (□), in Rotenone and DNOC experiments are underlined (—), in Rotenone and Diclofenac experiments are dashed (–-), and in DNOC, and Diclofenac experiments are labelled by name only.
