Table of Contents
International Journal of Proteomics
Volume 2010 (2010), Article ID 630134, 13 pages
http://dx.doi.org/10.1155/2010/630134
Research Article

Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

Department of Bioanalytical Ecotoxicology, Helmholtz Centre for Environmental Research-UFZ, Permoser Straße 15, 04318 Leipzig, Germany

Received 18 February 2010; Revised 26 May 2010; Accepted 19 July 2010

Academic Editor: Uwe Völker

Copyright © 2010 Karen Hanisch et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table 1: Identification of protein spots in the protein pattern of 5 days old zebrafish larvae.

Table 2: Protein spots changed in expression after exposure to EC10 of rotenone, DNOC and diclofenac in two or all three exposure experiments.

Table 3: Regulated protein spots after exposure of 3 days old zebrafish eleutheroembryos for 48 h to the EC10 of rotenone. 207 spots changed in expression, 116 of these protein spots were up-regulated and 91 down-regulated.

Table 4: Regulated protein spots after exposure of 3 days old zebrafish eleutheroembryos for 48 h to the EC10 of DNOC. 76 spots changed in expression, 56 of these protein spots were up-regulated and 20 down-regulated.

Table 5: Regulated spots after exposure of 3 days old zebrafish eleutheroembryos for 48 h to the EC10 of diclofenac. 47 spots changed in expression, 42 of these protein spots were up-regulated and 5 down-regulated.

Figure SM1: Comparison of the proteome pattern of eleutheroembryos of the zebrafish exposed to rotenone (A) DNOC (B) and diclofenac (C) with the respective EC10 concentration (rotenone = 0.05 μM; DNOC = 2.80 μM; Diclofenac = 12,6 μM). Only in expression upregulated proteins are depicted. The x-axis shows the pH-range and the y-axis the molecular weight (MW) in kDa. Exposed to rotenone, 116 proteins, to DNOC, 56 proteins and to Diclofenac, 41 proteins are increased in their intensity. Modified proteins are encircled and labelled with numbers. The labelled spots can be found in the supplementary material section.

Figure SM2: Comparison of the proteome pattern of eleutheroembryos of the zebrafish exposed to rotenone (A), DNOC (B) and diclofenac (C) with the respective EC10 concentration (rotenone = 0.05 μM; DNOC = 2.80 μM; diclofenac = 12,6 μM). Only repressed proteins are depicted. The x-axis shows the pH-range and the y-axis the molecular weight (MW) in kDa. Exposed to rotenone, 91 proteins, to DNOC, 20 proteins and to diclofenac, 14 proteins are decreased in their intensity. Modified proteins are encircled and labelled with numbers. The labelled spots can be found in supplementary material section.

  1. Supplementary Tables
  2. Supplementary Figures