Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

Table 1

Summary of results from spot-to-spot analysis for all performed toxicoproteomics experiments: Diclofenac (EC₁₀), Rotenone (EC₁₀) and DNOC (EC₁₀). Numbers and percentage (in relation to total protein number on the gel) of significantly (𝑃<.05) and highly-significantly (𝑃<.01) at least twofold changed proteins are indicated in the table. Data are sorted for up-regulated (oe, ratio sample/control >2) and down-regulated (rp, ratio sample/control <2) proteins. In addition, the sum or percentage of all changed proteins for each condition is shown (rp and oe).

Toxi Compound	Total number of Proteins detected on the gels	P value	rp [number of proteins]	rp [%]	oe [number of proteins]	oe [%]	rp and oe [number of proteins]	rp and oe [%]
Diclofenac	814	0.05	14	1.7	41	5.0	55	6.8
	814	0.01	4	0.5	15	1.8	19	2.3
Rotenone	860	0.05	91	10.6	116	13.5	207	24.1
	860	0.01	39	4.5	68	8.4	107	12.9
DNOC	703	0.05	20	2.8	56	8.0	76	10.8
	703	0.01	5	0.7	17	2.4	22	3.1