Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

Table 2

Summary of positive identification of protein spots changed in expression after exposure to EC₁₀ concentrations of Rotenone (R), DNOC (D) and Diclofenac (Dic) to zebrafish eleuthero-embryos.

Spot	Mascot Score	Protein Name	UniProtKB Accession	Peptides assigned	Sequence coverage [%]	MWobs/cal [kDa]	pIobs/cal	Ratio Rotenone	Ratio DNOC	Ratio Diclofenac
Dic11o, D, R	430.29	vitellogenin 1	Q1LWN2	12	8.7	30.0/128.0	5.6/8.68	13.6	4.1	2.4
Dic14o, D, R	94.0	LOC553473 (𝛽-crystallin)	Q502C7	5	10.8	24.5/27.6	5.9/7.67	47.3	3.1	3.0
Dic27o, D, R	197.79	actin, alpha, cardiac muscle 1a	Q6IQR3	10	23.1	45.0/42.0	5.6/5.22	3.3	3.6	2.3
Dic47o, D, R	644.34	tubulin, beta 2c	Q6P5M9	25	25.8	44.0/49.8	5.5/4.79	40.6	12	26.5
Dic64r, D, R	321.38	myosin, light polypeptide 2	O93409	14	50.3	17.0/18.9	4.7/4.39	0.4	0.5	0.5