Expression of Heat Shock and Other Stress Response Proteins in Ticks and Cultured Tick Cells in Response to Anaplasma spp. Infection and Heat Shock

Figure 2

2D DIGE analysis of the total proteome of uninfected and A. marginale-infected I. scapularis IDE8 tick cells showing GST underexpression in infected cells [5]. (a) 2D representative maps of uninfected (left) and infected (right) IDE8 cells that were labeled with Cy3 and Cy5, respectively. Circled spots correspond to proteins that were differentially expressed after infection. (b, c) Amplification of gel area where GST protein was localized after MS identification in uninfected (green square) and infected (red square) IDE8 cells. (d) 2D DIGE gel image corresponding to the overlapping Cy3 and Cy5 fluorochromes for uninfected versus infected paired samples in the GST region mentioned above.