Signatures of Drug Sensitivity in Nonsmall Cell Lung Cancer

Figure 1

Profiling of the activated (p-Tyr) RTK signaling pathways in the lung tumor cell lines. (a) Slide images of the CEER assay obtained from the eight tumor cell lines (please see the supplemental data and methods for experimental details available online at doi: 10.1155/2011/215496). (b) Graphical representation of the activated (p-Tyr) RTK pathways in the lung tumor cell lines profiled by the CEER assay. The cells were cultured in the presence of 10% FBS in their respective medium until 80% confluence and cell lysates were prepared in lysis buffer. The activation signal was determined from the harvested lysate. In each cell line the activated pathways are shown in RFU (relative fluorescent units) based on the lysate obtained from the number of cells being assayed.