Proteomic Approach to Evaluate Mechanisms That Contribute to Food Allergenicity: Comparative 2D-DIGE Analysis of Radioallergosorbent Test Positive and Negative Patients

Figure 2

SYPRO ruby-stained 2-D gel image of a 2D-DIGE separation of RAST⁺ and RAST⁻ samples. 2-D electrophoresis was performed using 24 cm, pH 3–10 linear IPG strips and 10% SDS-PAGE gels. Spots that showed statistically significant differences in intensity between the RAST⁻ and RAST⁺ are marked. Differences in Cye Dye-labeled sample abundances were analyzed using DeCyder Software. Differentially expressed protein spots with their respective fold differences and 𝑃 values are given as a table. A paired t-test derived 𝑃 value of ≤0.05 was considered statistically significant. Protein spots were excised and identified using MALDI-TOF and the identified proteins are listed in Table 2.