Proteomic Approach to Evaluate Mechanisms That Contribute to Food Allergenicity: Comparative 2D-DIGE Analysis of Radioallergosorbent Test Positive and Negative Patients

Figure 6

The biochemical processes networks of the differentially expressed proteins generated by MetaCore software using a transcription regulation algorithm. The biochemical processes regulation networks were ranked by a 𝑃 value and interpreted in terms of GO. Gene network illustrates proteins and interactions of the differentially expressed proteins in RAST⁺ and RAST⁻ patients. The network was obtained using analyze network algorithm of MetaCore and illustrates the close clustering and interconnectedness of biochemical process networks of the identified differentially regulated proteins in food allergy. Colored highlighted symbols (nodes) represent proteins (enzymes (yellow arrows), transporters (purple shapes), receptor ligands (green shapes), transfactors (red shapes), and other proteins (blue shapes)). Red solid circles correspond to differentially expressed proteins in RAST⁺ samples identified by MALDI analysis. Blue circles show the proteins in the network within the immediate vicinity of those identified proteins. The small colored hexagons on vectors between nodes describe positive interaction (green), unspecified interactions (black), or logical relationships (blue). Each connection (vector) represents a direct interaction between proteins.