Proteomic Approach to Evaluate Mechanisms That Contribute to Food Allergenicity: Comparative 2D-DIGE Analysis of Radioallergosorbent Test Positive and Negative Patients
Table 1
Lists of the clinical and demographic information for subjects enrolled in the study. * Denotes in mm the measure of wheal/flare in a antigen-specific skin test done relative to a saline control.
(a) Food rast negative patients.
Pt number
Age
Gender
Ethnicity
Clinical H/O
Skin test
RAST Antigen-specific IgE (ng/mL)
1
3
F
White
Peanut: urticaria and rash after peanut food challenge
Negative: peanut 2/4*
Negative: peanut and tree nuts
2
15
F
Black
Tree nut: anaphylaxis (angioedema, shortness of breath and abdominal pain)
Not done
Negative: peanut and tree nuts
3
1
M
White
Eczema workup
Positive: milk 12/20*
Negative: egg, milk and wheat
4
2
M
Hispanic
Eczema workup
Negative: egg 5/6*
Negative: egg
5
3
M
Black
Fish: urticaria
Positive: mixed Whitefish
Negative: tuna, salmon, cod
6
8
F
White
Cocoa: headache, strawberry: rash
Positive: cocoa 5/45* Negative: fresh strawberry
Negative: cocal and strawberry
7
7
M
Not specified
Peanut: hives, egg: hives
Not done
Negative: egg, peanut and tree nuts
8
7
F
White
Tuna: hives
Negative: tuna 4/5* (negative)
Negative: tuna
9
9
M
Not specified
Tomato: facial edema
Not done
Negative: tomato
(b) Food rast positive patients.
Pt number
Age
Gender
Ethnicity
Clinical H/O
Skin test
RAST Antigen-specific IgE (ng/mL)
1
6
M
Black
Peanut: angioedema, shortness of breath and itchy eyes
Not done
Peanut: >100
2
4
M
Black
Peanut: rash
Positive: peanut (5/20)*
Peanut: 0.86
3
5
F
Black
Peanut: hives, soy: eczema flair
Positive: soy (5/10) and egg (3/7)
Peanut: 7.32, soy: 5.15 and tree nuts: 4.14–>100
4
3
M
Black
Peanut: hives
Positive: peanut (12/20)*
Peanut: 72.8
5
2
F
White
Peanut: eczema flare
Positive: peanut (15/40)*
Peanut: 4.07
6
6
M
White
Tree nut: difficulty breathing
Not done
Tree nuts: 15–>100
7
16
M
Black
Crab: angioedema
Positive: crab (10/12)*, lobster (10/13)* and clam (4/6)*
