Proteomic Approach to Evaluate Mechanisms That Contribute to Food Allergenicity: Comparative 2D-DIGE Analysis of Radioallergosorbent Test Positive and Negative Patients

Table 2

List of the up/down regulated proteins from RAST⁺ and RAST⁻ samples analyzed by 2D-DIGE and further identified by MALDI-TOF. Data shown in the table include spot number, calculated mass, theoretical pI, number of matching peptides, % sequence coverage, name and symbol of identified proteins.

Spot number	Mass	𝑃I value	Peptides matches	Sequence coverage (%)	Protein name	Symbol
189	14505	6.81	7	59	40S ribosomal protein S12	RS12_HUMAN
1244	54005	6.21	11	28	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4	F264_HUMAN
1008	35830	6.51	7	32	Aldose reductase	ALDR_HUMAN
1213	15683	5.42	6	27	Cellular retinoic acid-binding protein 2	RABP2_HUMAN
1302	61312	8.63	8	17	Chondroitin sulfate N-acetylgalactosaminyltransferase 1	CGAT1_HUMAN
1387	91751	5.98	15	19	Coiled-coil domain-containing protein	CF204_HUMAN
1299	83214	7.23	8	17	Complement C2	CO2_HUMAN
1428	36702	8.45	10	39	C-type lectin domain family 4 member K	CLC4K_HUMAN
963	38901	5.27	9	26	DDB1- and CUL4-associated factor 7	DCAF7_HUMAN
1105	40517	9.00	10	28	Developmentally regulated GTP-binding protein 1	DRG1_HUMAN
1225	30836	8.71	6	18	DnaJ homolog subfamily C member 27	DJC27_HUMAN
416	16373	10.08	7	59	DnaJ(HSP40) homolog	DJC15_HUMAN
944	77221	5.36	11	21	Elongation factor G 2	EFG2_RALME
1448	104682	6.08	12	12	Exostosin-like 3	EXTL3_HUMAN
105	81024	6.45	13	20	FACT complex subunit SSRP1	SSRP1_HUMAN
661	16459	8.67	6	51	Globin-1 OS=Petromyzon marinus PE=1 SV=2	GLB1_PETMA
1309	37162	10.80	8	31	Heparan sulfate glucosamine 3-O-sulfotransferase6	HS3S6_HUMAN
1080	57528	6.12	14	22	IKAROS zinc finger protein 1	IKZF1_HUMAN
1513	18033	9.15	6	49	Interleukin-17F	IL17F_HUMAN
980	129489	7.14	11	12	Laminin subunit beta-3	LAMB3_HUMAN
403	36288	7.08	8	24	L-lactate dehydrogenase C chain	LDHC_HUMAN
242	47983	9.64	9	23	MAPK/MAK/MRK overlapping kinase	MOK_HUMAN
1132	21215	9.03	7	24	Mast cell expressed membrane protein 1	MCEM1_HUMAN
684	181570	5.94	13	17	Mitogen-activated protein kinase kinase kinase 4	M3K4_HUMAN
907	121648	9.46	11	14	Myosin-Ic	MYO1C_HUMAN
1191	60539	9.62	19	28	Parafibromin	CDC73_HUMAN
1187	43939	9.33	10	24	Pentatricopeptide repeat-containing protein 2	PTCD2_HUMAN
1090	56852	8.52	10	21	Probable histidyl-tRNA synthetase, mitochondrial	SYHM_HUMAN
761	50550	5.00	10	34	Rab GDP dissociation inhibitor alpha	GDIA_HUMAN
983	79767	5.54	13	18	RUN and FYVE domain-containing protein 1	RUFY1_HUMAN
981	12439	10.54	7	73	Somatoliberin	SLIB_HUMAN
763	33047	6.64	7	31	Tissue factor	TF_HUMAN
918	23294	11.33	7	44	Transcription factor 23	TCF23_HUMAN
1264	64218	8.56	9	23	Transmembrane anterior posterior transformation protein 1 homolog	TAPT1_HUMAN
1510	121711	5.95	16	22	Transmembrane protein 132C OS	T132C_HUMAN
1432	23069	9.51	6	38	UPF0684 protein C5orf30	CE030_HUMAN
1367	57986	6.11	8	19	Zinc finger protein Aiolos	IKZF3_HUMAN