Research Article

Miniaturized Mass-Spectrometry-Based Analysis System for Fully Automated Examination of Conditioned Cell Culture Media

Figure 1

Schematic of miniaturized cell culture chamber: a modified 2 mL glass vial. Five fused silica tubings inserted through perforated cap provide liquid in- and outlets. Endings of fused silica tubings are fitted with a porous frit to prevent cells from leaving the vial and contaminating the analysis system. Overpressure of approximately 6.5 * 105 Pa inside chamber operates as pumping system. Efficient mixing of culture is ensured by minimagnet at vial bottom in combination with underlying magnetic stirrer. Light path for OD determination goes straight through vial between magnet and endings of fused silica.
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