Research Article

Miniaturized Mass-Spectrometry-Based Analysis System for Fully Automated Examination of Conditioned Cell Culture Media

Figure 2

Overall analysis setup incorporating two syringes for MM and solvent supply and a switching valve for alternating between concentration/accumulation and elution steps. Cell culture chamber, placed on a magnetic stirrer, is connected via six-port valve with one of three C4 columns, which maintain a stable system backpressure. Via an electrospray needle held at 1.2 kV, sample is deposited onto a MALDI target plate.
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