Rapid Screening of the Epidermal Growth Factor Receptor Phosphosignaling Pathway via Microplate-Based Dot Blot Assays
Figure 3
Dot blot assay feasibility—titration of pure GAPDH. (a) The titration curve demonstrates a range 4–100 ng input protein whereby linear signal could be detected. Each point represents the mean of 4 replicates. (b) Coomassie staining of membrane wells after protein loading. Four replicates are shown for each concentration point.
