The Application of a Three-Step Serum Proteome Analysis for the Discovery and Identification of Novel Biomarkers of  Hepatocellular Carcinoma

<table>Representative chromatogram during removal of highly abundant serum proteins by an immunoaffinity column. 100 <i>μ</i>L of serum (diluted fivefold) was injected on the immunoaffinity column and was eluted (0.5 mL/min) as described in the experimental procedures. Flow-through fractions (12.1–20.0 min) were collected, and then a total of 4.0 mL fractions were concentrated to a volume of 80 <i>μ</i>L using Vivaspin 2 for reversed-phase HPLC fractionation.</table>

International Journal of Proteomics

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Figure 2

Figure 2: The Application of a Three-Step Serum Proteome Analysis for the Discovery and Identification of Novel Biomarkers of  Hepatocellular Carcinoma 

Figure 2 | The Application of a Three-Step Serum Proteome Analysis for the Discovery and Identification of Novel Biomarkers of  Hepatocellular Carcinoma 