Research Article

The Application of a Three-Step Serum Proteome Analysis for the Discovery and Identification of Novel Biomarkers of Hepatocellular Carcinoma

Figure 6

Western blot analysis of clusterin in sera from HCC and LC groups. (a) Immunoedpleted sera of the 5 HCC and 5 LC SDS-PAGE cases and additional 5 HCC and 5 LC cases were separated by 10.0–12.0% SDS-PAGE and probed with anticlusterin. The expression levels are relatively higher in the HCC groups than LCs. (b) Differences in expression were analyzed by the Student’s -test. The expression levels of these proteins were upregulated significantly in HCC samples ( ). Rhombuses represent volumes of individual samples. Line indicates the range with the open circles indicating the mean values.
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(a)
623190.fig.006b
(b)