An Economical High-Throughput Protocol for Multidimensional Fractionation of Proteins

Figure 3

The distribution of cytosolic proteins, extracted from twelve samples of cultured human Neuro-2A cells, in anion-exchange chromatographic fractions. Proteins were quantitated using a BCA method and quantities were normalised as a percentage of total extracted protein. The flow-through (grey), 0.15 M (red), 0.25 M (green), 0.4 M (violet) and 1.0 M (blue) potassium chloride eluate chromatographic fractions display high between sample precision using the anion-exchange protocol described.