Method for Recovery and Immunoaffinity Enrichment of Membrane Proteins Illustrated with Metastatic Ovarian Cancer Tissues
Figure 1
2D gel analysis of proteins extracted from the same metastatic ovarian tumor sample by (a) HEPES buffer (Gel 1) and (b) TD2 buffer (Gel 2). Each extraction was conducted for 30 pressure cycles (30,000 psi for 20 sec followed by 0 psi for 20 sec) at a concentration of 75 mg tissue/mL of extraction buffer using the Barocycler. The samples were subsequently diluted to 8.6 mg/mL in ProteoSolve-IEF (a denaturing IEF gel buffer) for gel analysis. Automated gel image analysis of the Coomassie-stained gels suggests that 97% of the protein spots are shared between the two gels in position with most (74%) of the same abundance (d). One spot is uniquely found in the HEPES control, and 14 spots are unique to the ProteoSolve-TD2 extraction.
