Figure 3: Misregulated proteins of mechanistic interest in FASD were confirmed with Western blotting. Both alcohol and control protein (20 μg) samples were run on SDS-PAGE gels and detected by chemical luminescence. GAPDH was used as an internal control. Statistical analysis of band density by Student’s -test showed that alcohol upregulates serotransferrin (Tf) and triosephosphate isomerase (Tpi1) ( ).