Table of Contents
International Journal of Proteomics
Volume 2012, Article ID 980829, 9 pages
http://dx.doi.org/10.1155/2012/980829
Research Article

Reconstruction of Sugar Metabolic Pathways of Giardia lamblia

1Institute of Molecular BioSciences, Massey University, Palmerston North 4442, New Zealand
2Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand

Received 2 August 2012; Revised 18 September 2012; Accepted 23 September 2012

Academic Editor: Vladimir Uversky

Copyright © 2012 Jian Han and Lesley J. Collins. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Giardia lamblia is an “important” pathogen of humans, but as a diplomonad excavate it is evolutionarily distant from other eukaryotes and relatively little is known about its core metabolic pathways. KEGG, the widely referenced site for providing information of metabolism, does not yet include many enzymes from Giardia species. Here we identify Giardia’s core sugar metabolism using standard bioinformatic approaches. By comparing Giardia proteomes with known enzymes from other species, we have identified enzymes in the glycolysis pathway, as well as some enzymes involved in the TCA cycle and oxidative phosphorylation. However, the majority of enzymes from the latter two pathways were not identifiable, indicating the likely absence of these functionalities. We have also found enzymes from the Giardia glycolysis pathway that appear more similar to those from bacteria. Because these enzymes are different from those found in mammals, the host organisms for Giardia, we raise the possibility that these bacteria-like enzymes could be novel drug targets for treating Giardia infections.