Proteomic Analysis of the Ontogenetic Variability in Plasma Composition of Juvenile and Adult Bothrops jararaca Snakes

Table 2

Identification of spots showing quantitative variation in juvenile and adult B. jararaca plasma indicated in Figure 2, by ESI-Q-TOF (MS/MS).

(a)  

Juvenile Bothrops jararaca snake
Spot number^a	Protein name (organism)	Score	Protein accession number^b	Peptide sequences^c	Volume^d (%)
1	γ-phospholipase A2 inhibitor; (Bothrops jararaca)	556	gi∣157885066	KCIDIVGHR; KNCFSSSICKL; SCDFCHNIGK; VFLEISSASLSVR; HEHFPGDIAYNLK; LGQIDVNIGHHSYIR; DCDGYQQECSSPEDVCGK; CIDIVGHRHEHFPGDIAYNLK	1.6137
14	γ-phospholipase A2 inhibitor subunit B; (Trimeresurus flavoviridis)	120	gi∣155676753	RACCVGDECK; GCATESLCTLLQK	1.6347
16	γ-phospholipase A2 inhibitor; (Bothrops jararaca)	467	gi∣157885066	KCIDIVGHR; NCFSSSICK; VFLEISSASLSVR; HEHFPGDIAYNLK; TVHKNCFSSSICK; LGQIDVNIGHHSYIR; DCDGYQQECSSPEDVCGK; KCIDIVGHRHEHFPGDIAYNLK	0.5292
20	γ-phospholipase A2 inhibitor subunit B; (Trimeresurus flavoviridis)	284	gi∣155676753	ACCVGDECK; RACCVGDECK; DTENQCLSLTGK; GCATESLCTLLQK	0.5687
22	γ-phospholipase A2 inhibitor; (Bothrops jararaca)	496	gi∣157885068	INCCEK; KCIDIVGHR; GRINCCEK; NCFSSSICK; VFLEISSASLSVR; HEHFPGDIAYNLK; TVHKNCFSSSICK; LGQIDVNIGHHSYIR; KCIDIVGHRHEHFPGDIAYNLK	3.5287
39	C3 complement; (Naja naja)	221	gi∣399269	RVGLVAVDK; IWDTIEK; IQKPGAAMK; GIYTPGSPVR; IKLEGDPGAR; AVYVLNDKYK; EYVLPSFEVR	0.1644
44	Albumin; (Trimeresurus flavoviridis)	92	gi∣56790036	LVEDIQNDHIIQ; IIPQAPTSNLIEITKR	0.3461

(b)  

Adult Bothrops jararaca snake
Spot number^a	Protein name (organism)	Score	Protein accession number^b	Peptide sequences	Volume^c (%)
7	γ-phospholipase A2 inhibitor; (Bothrops jararaca)	511	gi∣157885066	KCIDIVGHR; NCFSSSICK; VFLEISSASLSVR; HEHFPGDIAYNLK; LGQIDVNIGHHSYIR; DCDGYQQECSSPEDVCGK; KCIDIVGHRHEHFPGDIAYNLK	0.5813
16	γ-phospholipase A2 inhibitor; (Bothrops jararaca)	470	gi∣157885066	KCIDIVGHR; NCFSSSICK; SCDFCHNIGK; VFLEISSASLSVR; HEHFPGDIAYNLK; LGQIDVNIGHHSYIR; DCDGYQQECSSPEDVCGK	0.0990
18	Transferrin; (Lamprophis fuliginosus)	220	gi∣108792441	IVWCAVGK; VCTFHTHDW; EADAITLDGGHIYTAGK	0.4829
24	Transferrin; (Lamprophis fuliginosus)	232	gi∣108792441	LVLEQQK; IVWCAVGK; VCTFHTHDW; EADAITLDGGHIYTAGK	0.2124
29	C3 complement; (Naja naja)	363	gi∣399269	VGLVAVDK; LEGDPGAR; IWDTIEK; IQKPGAAMK; GIYTPGSPVR; IKLEGDPGAR; DTCMGTLVVK; AVYVLNDKYK; EYVLPSFEVR	0.2256
38	𝛼-phospholipase A2 inhibitor precursor; (Bothrops jararaca)	383	gi∣167547111	LYVTNK; REFANLR; KNFEALR; RSFGSGSER; GAFLTVHKA; KAFANVLER; KVLNSLIDALMHLQRE; QICEQAEGHIPSPQLENHNK	0.1067
40	β-Actin; (Rachycentron canadum)	867	gi∣161376754	AGFAGDDAPR; DLTDYLMK; GYSFTTTAER; EITALAPSTMK; AVFPSIVGRPR; DSYVGDEAQSKR; IWHHTFYNELR; QEYDESGPSIVHR; LDLAGRDLTDYLMK; SYELPDGQVITIGNER; EEEIAALVVDNGSGMCK; VAPEEHPVLLTEAPLNPK; DLYANTVLSGGTTMYPGIADR; TTGIVMDSGDGVTHTVPIYEGYALPHAILR	0.0060
41	Antihemorrhagic factor Bj46a (Bothrops jararaca)	68	gi∣48428681	YALNVIKN; EGHAHSHLIQQHVEK; NCPKCPILLPSNNPQVVDSVEYVLNKHNEK; HNEKLSDHVYEVLEISR; GDLECDEKDAKEWTDTGVR; IMFNVDTFKEDVFAK; LSDHVYEVLEISR; VPVAFVK; ELPKDISDR; VHHFEL; EWTDTGVR	0.09264
43	Albumin; (Trimeresurus flavoviridis)	234	gi∣56790036	ECFDTK; YGINDCCAK; LVEDIQNDHIIQ; QLCHCCDSSFISR; LEDHVQCLHTGEEQLK	0.0497
44	Albumin; (Trimeresurus flavoviridis)	235	gi∣56790036	FIETHEK; NNCDNYK; LVEDIQNDHIIQ; QLCHCCDSSFISR; LEDHVQCLHTGEEQLK	0.3964

^aSpot number refers to that shown in Figure 2.
^bNCBI accession number.
^cObtained by MS/MS analysis.
^d𝑃<0.05.