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Authors/year | Specimen | Quantitative approach | Sample preparation | Outcomes |
|
Yang et al. 2011 [46] | Urine 54 bladder cancer patients, 46 controls | Label-free—spectral count | NIL | Quantified 265 glycoproteins. alpha-1-antitrypsin, 74% sensitivity and 80% specificity for bladder cancer patients. |
Quintana et al. 2009 [47] | Urine 39 patients kidney chronic allograft dysfunction, 32 controls | Label-free—peak peptide intensity | SCX using magnetic beads | Peptides from uromodulin and kininogen significantly elevated in control compared to CAD patients. |
Hanas et al. 2008 [48] | Serum 13 pancreatic adenocarcinoma patients, 12 healthy controls
| Label-free—peak peptide intensity | NIL | Quantified 20 low-mass serum peaks. Bootstrap analysis showed peaks could differentiate cancer from control sera with 95% accuracy. |
Xue et al. 2010 [49] | Cell lysates Primary and lymph node metastatic cell lines. 1 patient. | Label-free—peak peptide intensity | NIL | 145 differential proteins. Western blot and ROC curve analysis confirmed that 2 specific proteins could predict colorectal cancer metastasis. |
Besson et al. 2011 [50] | Colorectal cancer tissue 28 colorectal frozen tissue samples
| Stable isotope labeling—iTRAQ | Peptide OFFGEL fractionation | 555 proteins with significant fold change between different cancer stages. Identified a candidate with increased abundance in adenomas and early stage colorectal cancer. |
Bondar et al. 2007 [51] | Serum 6 healthy male, 20 nonmalignant prostate biopsy patients, 26 malignant prostate cancer patients | Stable isotope labeling | NIL | Higher abundance of Zn-α2 glycoprotein (ZAG) in prostate cancer patients than nonmalignant prostate disease patients and healthy controls. |
Chaerkady et al. 2008 [52] | Liver tissue 55 samples of hepatocellular carcinoma, 20 samples of adjacent noncancer tissues | Stable isotope labeling—iTRAQ | SCX | 59 proteins increased in abundant, 92 proteins were less abundant in HCC compared to normal tissue. 12 proteins further validated using immunohistochemical labeling. |
Dayon et al. 2008 [53] | Cerebrospinal fluid 4 postmortem CSF patients, 4 antemortem CSF from living healthy controls | Stable isotope labeling—tandem mass tag isobaric labeling | Immunoaffinity depletion of 6 most abundant proteins and SCX | 78 proteins more abundant in postmortem samples compared to antemortem. |
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