International Journal of Proteomics / 2013 / Article / Tab 2

Review Article

Current Status and Advances in Quantitative Proteomic Mass Spectrometry

Table 2

Recent quantitative MS-based studies involving human samples.

Authors/yearSpecimenQuantitative approachSample

Yang et al. 2011 [46]Urine
54 bladder cancer patients, 46 controls
Label-free—spectral countNILQuantified 265 glycoproteins. alpha-1-antitrypsin, 74% sensitivity and 80% specificity for bladder cancer patients.
Quintana et al. 2009 [47]Urine
39 patients kidney chronic allograft dysfunction, 32 controls
Label-free—peak peptide intensitySCX using
magnetic beads
Peptides from uromodulin and kininogen significantly elevated in control compared to CAD patients.
Hanas et al. 2008 [48]Serum
13 pancreatic adenocarcinoma patients, 12 healthy controls
Label-free—peak peptide intensityNILQuantified 20 low-mass serum peaks. Bootstrap analysis showed peaks could differentiate cancer from control sera with 95% accuracy.
Xue et al. 2010 [49]Cell lysates
Primary and lymph node metastatic cell lines. 1 patient.
Label-free—peak peptide intensityNIL145 differential proteins. Western blot and ROC curve analysis confirmed that 2 specific proteins could predict colorectal cancer metastasis.
Besson et al. 2011
Colorectal cancer tissue
28 colorectal frozen tissue samples
Stable isotope labeling—iTRAQPeptide
555 proteins with significant fold change between different cancer stages. Identified a candidate with increased abundance in adenomas and early stage colorectal cancer.
Bondar et al. 2007 [51]Serum
6 healthy male, 20 nonmalignant prostate biopsy patients, 26 malignant prostate cancer patients
Stable isotope labelingNILHigher abundance of Zn-α2 glycoprotein (ZAG) in prostate cancer patients than nonmalignant prostate disease patients and healthy controls.
Chaerkady et al. 2008 [52]Liver tissue
55 samples of hepatocellular carcinoma, 20 samples of adjacent noncancer tissues
Stable isotope labeling—iTRAQSCX 59 proteins increased in abundant, 92 proteins were less abundant in HCC compared to normal tissue. 12 proteins further validated using immunohistochemical labeling.
Dayon et al. 2008
Cerebrospinal fluid
4 postmortem CSF patients, 4 antemortem CSF from living healthy controls
Stable isotope labeling—tandem mass tag isobaric labeling Immunoaffinity depletion of 6
most abundant
proteins and SCX
78 proteins more abundant in postmortem samples compared to antemortem.