iTRAQ-Based and Label-Free Proteomics Approaches for Studies of Human Adenovirus Infections
Expression analysis of five cellular proteins and comparison of different quantitation methods. Lysates from Ctrl (noninfected cells) and HAdV-B3/C5-infected A549 cells (biological duplicates) were used for Western blot analyses. Alpha-tubulin was used as a loading control for the normalization. Western blot analyses and quantitative comparisons were performed for Gal1 (LEG1_HUMAN) (a), Gal3 (LEG3_HUMAN) (b), AG2 (AGR2_HUAMN) (c), and CD98 (4F2_HUMAN) (d). nq: no quantitation value was obtained.