Additions to the Human Plasma Proteome via a Tandem MARS Depletion iTRAQ-Based Workflow
Figure 1
(a) The iTRAQ-based quantitative platform used for plasma proteome analyses in which the flow-through fractions from four crude plasma samples (A–D) are modified with iTRAQ 4-plex reagents, pooled into a single mixture, and separated with offline SCX-LC-MS/MS. Example chromatograms () from three independent injections of plasma sample C upon (b) MD and (c) TMD are shown.
