Issues and Applications in Label-Free Quantitative Mass Spectrometry

Figure 5

Impact of brain saline perfusion (Exp. 2) to reduce blood-protein contamination and expand the LC gradient by 50% (Exp. 2) on protein abundance among the top 5 pituitary proteins identified and quantified in the two experiments. WT: wild type mouse; KI: 𝐿ℎ𝑥3^{𝑊227𝑡𝑒𝑟/𝑊227𝑡𝑒𝑟} knock-in mouse; *𝑃<0.00876, 𝑞<0.05.

(a)  

(b)