Table of Contents
International Journal of Proteomics
Volume 2014, Article ID 395905, 10 pages
Research Article

Comparative Analysis of Sorghum bicolor Proteome in Response to Drought Stress and following Recovery

1Institute of Ecology, Evolution, and Diversity, Johann Wolfgang Goethe-University Frankfurt, Max-Von-Laue Straße 13, 60438 Frankfurt, Germany
2Biodiversity and Climate Research Centre (BiK-F), 60325, Frankfurt am Main, Germany
3Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza 12619, Egypt
4Institute of Pharmaceutical Chemistry, Johann Wolfgang Goethe-University Frankfurt, 60438 Frankfurt, Germany

Received 30 June 2014; Revised 8 September 2014; Accepted 8 September 2014; Published 1 October 2014

Academic Editor: Michael Hippler

Copyright © 2014 Christoph Jedmowski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The adaptive response of Sorghum bicolor landraces from Egypt to drought stress and following recovery was analyzed using two-dimensional difference gel electrophoresis, 2D-DIGE. Physiological measurements and proteome alterations of accession number 11434, drought tolerant, and accession number 11431, drought sensitive, were compared to their relative control values after drought stress and following recovery. Differentially expressed proteins were analysed by Matrix assisted laser desorption ionisation time-of-flight mass spectrometry, MALDI-TOF-MS. Alterations in protein contents related to the energy balance, metabolism (sensu Mewes et al. 1997), and chaperons were the most apparent features to elucidate the differences between the drought tolerant and sensitive accessions. Further alterations in the levels of proteins related to transcription and protein synthesis are discussed.