Table of Contents
International Journal of Proteomics
Volume 2014 (2014), Article ID 395905, 10 pages
http://dx.doi.org/10.1155/2014/395905
Research Article

Comparative Analysis of Sorghum bicolor Proteome in Response to Drought Stress and following Recovery

1Institute of Ecology, Evolution, and Diversity, Johann Wolfgang Goethe-University Frankfurt, Max-Von-Laue Straße 13, 60438 Frankfurt, Germany
2Biodiversity and Climate Research Centre (BiK-F), 60325, Frankfurt am Main, Germany
3Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza 12619, Egypt
4Institute of Pharmaceutical Chemistry, Johann Wolfgang Goethe-University Frankfurt, 60438 Frankfurt, Germany

Received 30 June 2014; Revised 8 September 2014; Accepted 8 September 2014; Published 1 October 2014

Academic Editor: Michael Hippler

Copyright © 2014 Christoph Jedmowski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Aliquots of 50 µg of protein samples extractions were labeled with Cy-2, control, Cy-3, drought treatment or Cy-5, recovery treatment, using Cy-Dye DIGE Fluor Minimal Dye Labeling Kit (GE Healthcare) following the manufacturer’s recommendation. Following electrophoresis as described in the materials and methods part, gels were scanned using a Typhoon 9410 scanner (GE healthcare). Images were analyzed using DeCyder 2-D Differential Analysis Software (GE healthcare) to identify up and downregulated proteins. Only proteins with 1.5-fold threshold change in relative fluorescence signal intensity are indicated and further subjected for MALDI-TOF-MS analysis.

  1. Supplementary Figure