Comparative Analysis of Sorghum bicolor Proteome in Response to Drought Stress and following Recovery

Table 1

MALDI-TOF-MS results of picked spots from control, drought treatment and recovery of drought tolerant, # 11434, and susceptible, # 11431, genotypes and their expression values in comparison to control. The search carried out against the entire NCBInr database; SID: spot identification number; Mr/pI: calculated molecular weight and isoelectric point of predicted proteins; : Score; protein scores greater than 84 are significant (); %: percentage of coverage; : number of peptides matched; : drought; : recovery.

Spot ID	NCBI Acc. no.	Protein name	Mr/pI				# 11434		# 11431
							01: Metabolism
6	gi/18483235	Methionine  synthase/Sorghum  bicolor	84135/5.93	96	8%	8	6.7	1.6	6.6
7	gi/18483235	Methionine  synthase/Sorghum  bicolor	84135/5.93	102	12%	12			5.5
16	gi/226502947	S-adenosylmethionine  synthase  l  Z.  mays	42986/5.57	193	19%	10	1.7		1.5
29	gi/666089	P-(S)-hydroxymandelonitrile  lyase  Sorghum  bicolor	41796/4.72	154	13%	8			3.6
02: Energy
1	gi/242096062	Hypothetical  protein  Sorbidraft  of  S.  bicolor  homologues  to  putative  C4; phosphoenolpyruvate  carboxylase/Saccharum  officinarum  CAC08829.1	108988/5.89	306	29%	28	2.1		3.3	−2
2	gi/242096062	Hypothetical  protein  Sorbidraft  of  S.  bicolor  homologues  to  putative  C4; phosphoenolpyruvate  carboxylase/S.  officinarum  CAC08829.1	108988/5.89	504	30%	33			1.7
3	gi/242096062	Hypothetical  protein  Sorbidraft  of  S.  bicolor  homologues  to  putative  C4; phosphoenolpyruvate  carboxylase/S.  officinarum  CAC08829.1	108988/5.89	331	29%	30			2.3	−2
4	gi/242096062	Hypothetical  protein  Sorbidraft  of  S.  bicolor  homologues  to  putative  C4; phosphoenolpyruvate  carboxylase/S.  officinarum  CAC08829.1	108988/5.89	354	26%	30			2.4
5	gi/242080811	Hypothetical  protein  Sorbidraft  of  S.  bicolor  homologues; to  aconitate  hydratase  of  Oryza  sativa  Japonica  Q6YZX6.1	108402/6.41	101	11%	13			1.8
9	gi/242051769	Hypothetical  protein  Sorbidraft  of  S.  bicolor  homologues  to; chloroplast  NADP-dependent  malic  enzyme  Z.  mays  NP_001105313.1	69904/6.23	181	28%	21	−2.8	−2.4
10	gi/242051769	Hypothetical  protein  Sorbidraft  of  S.  bicolor  homologues  to; chloroplast  NADP-dependent  malic  enzyme  Z.  mays  NP_001105313.1	69904/6.23	199	25%	18	−2.8	−2.7
11	gi/242051769	Hypothetical  protein  Sorbidraft  of  S.  bicolor  homologues  to; chloroplast  NADP-dependent  malic  enzyme  Z.  mays  NP_001105313.1	69904/6.23	193	26%	18	−2.8	−2.3	−2
17	gi/242059597	Hypothetical  protein  Sorbidraft  of  S.  bicolor  homologues  to; fructose-1,6-bisphosphat  aldolase,  cytosol  Z.  mays  NP_001105336.1	38990/6.96	278	23%	12			1.6
23	gi∣195634659	Plastid  fructose-1,6-bisphosphate  aldolase/Z.  mays	41924/7.63	607	37%	22	−2.2	−1.9	−2.4
24	gi∣195634659	Plastid  fructose-1,6-bisphosphate  aldolase,  Z.  mays	41924/7.63	395	34%	19	−2.1	−1.9	−1.8
19	gi∣108705994	Plastid  glyceraldehyde-3-phosphate  dehydrogenase/O.  sativa  Japonica	34024/4.99	121	19%	11	−1.5
20	gi/255540341	Cytosolic  glyceraldehyde-3-phosphate  dehydrogenase/Ricinus  communis	36930/7.10	157	20%	14	1.6	1.7	1.7
30	gi/30385668	Pyruvate  phosphate  dikinase/Sorghum  bicolor	103021/5.68	94	16%	17	1.9	1.6
04: Transcription
21	gi/242085078	Hypothetical  protein  Sorbidraft  of  S.  bicolor  homologues  to; RNA-binding  protein/Arabidopsis  thaliana  NP_172405.1	42324/8.89	438	40%	25			−2
28	gi∣195637410	40S  ribosomal  protein  S3/Z.  mays	25605/9.4	117	46%	12		1.8
05: Protein synthesis
15	gi∣195620072	Elongation  factor  alpha/Z.  mays	49534/9.15	225	21%	14	8.5	5	7
06: Protein destination/storage
8	gi∣162459902	Nucleoredoxin  1/Z.  mays	64058/4.8	168	16%	12	4.4	4.9	4.5
12	gi/242094438	Hypothetical  protein  Sorbidraft  of   S.  bicolor  homologues  to  Heat  shock  protein  60	61927/5.47	148	31%	16		2.1
13	gi/242094438	Hypothetical  protein  Sorbidraft  of   S.  bicolor  homologues  to  Heat  shock  protein  60	61927/5.47	161	29%	14		2.2
14	gi/242094438	Hypothetical  protein  Sorbidraft  of   S.  bicolor  homologues  to  Heat  shock  protein  60	61927/5.47	126	19%	12	1.9	2.3
18	gi/242041951	Hypothetical  protein  Sorbidraft  of  S.  bicolor  homologues  to; pepsin/retropepsin  like  aspartat  protease/Z.  mays  ACG35399.1	42799/8.96	79	14%				2
31	gi/242056107	Hypothetical  protein  Sorbidraft  of  S.  bicolor  homologues  to; mitocondrial  processing  peptidase/Z.  mays  NP_001150614.1	54054/6.24	198	28%	12			1.7
32	gi/145666464	Protein  disulfide  isomerase/Z.  mays	56921/5.01	174	19%	18	2.1	2.5
12: Unclear classification
22	gi/242075782	Hypothetical  protein  Sorbidraft  of  S.  bicolor  homologues  to  ABA; stress- and  fruit-ripening  inducible-like  protein  Z.  mays  CAA72998.1	28466/4.92	258	65%	22	3.6	4.5	7
25	gi/242035869	Osr40Sc1  like  protein	39718/6.27	66	9%	3			5.2
26	gi/242035869	Osr40Sc1  like  protein	39718/6.27	201	13%	6	3.6	3.3	3
27	gi/242056773	Hypothetical  protein  Sorbidraft/S.  bicolor	31577/6.06	205	23%	9	3.6	2.9	4.6
33	gi/242095250	Hypothetical  protein  Sorbidraft/S.  bicolor	41333/4.91	222	37%	14	1.9	2.3