Quantitative Proteomics and Lipidomics Analysis of Endoplasmic Reticulum of Macrophage Infected with Mycobacterium tuberculosis

Figure 3

H37Rv inhibits apoptosis of THP-1 cells through caspase 8 and modulates cholesterol synthesis for latent infection. (a) Observed PIP2 levels were more than fivefold in THP-1 cells infected with H37Rv, compared to H37Ra which has direct effect on caspase 8. (b) Measured caspase activity in H37Rv infected THP-1 cells was one-half fold compared to H37Ra. Further caspase 9 activity remained unchanged. Data are representative of at least three independent experiments with similar results. (c) The cell lysates from UI, H37Ra, and H37Rv were analysed by western blot with anti-EST1 and anti-AMRP primary antibodies and β-actin was used as a loading control. (d) (i) Bands corresponding to EST-1 were quantified, and the intensities of each protein were normalized to the intensity of actin. (d) (ii) Similarly relative intensities of AMRP were normalized and quantified. White bar represents H37Rv while black bar represents H37Ra.

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