Quantitative Proteomics and Lipidomics Analysis of Endoplasmic Reticulum of Macrophage Infected with Mycobacterium tuberculosis

Table 1

Representation of the list of 18 ER proteins in THP-1 cells infected with either H37Ra or H37Rv.

	Accession number	Protein identity	Name	H37Ra	H37Rv	Functions
1	P16615	ATP2A2	Sarcoplasmic/endoplasmic reticulum calcium ATPase 2	0.0121	0.3839	Calcium homeostasis
2	P23141	EST1 or CES1	Liver carboxylesterase 1	1.6828	ND	Cholesterol homeostasis
3	P30533	AMRP	Alpha-2-macroglobulin receptor-associated protein	2.0374	ND	Cholesterol homeostasis
4	P14625	ENPL	Endoplasmin	0.9387	15.9431	Calcium homeostasis
5	P13667	PDIA4	Protein disulfide-isomerase A4	1.3674	21.4636	Termination of classical mitochondrial apoptotic pathway
6	P27824	CANX	Calnexin	1.2835	27.1939	Stops ER stress mediated apoptosis
7	P23284	PPIB	Peptidyl-prolyl cis-trans isomerase B	0.8023	10.6105	NF-κB pathway
8	P14314	GLU2B	Glucosidase 2 subunit beta	1.1434	16.7416	Novel interactor of IP3R
9	O94905	ERLN2	Erlin-2	1.2078	21.7272	ER associated degradation of activated IP3R
10	Q9UBR2	CTSZ	Cathepsin Z	1.0487	10.9698	Diminished activation and proliferation of T-lymphocytes
11	P04844	RPN2	Dolichyl-diphosphooligosaccharide	0.9903	ND	Unknown
12	P49755	TMED10	Transmembrane emp24 domain-containing protein 10	1.212	ND	Vesicle biogenesis, Cargo uptake
13	Q15084	PDIA6	Protein disulfide-isomerase A6	1.483	ND	Unfold protein response
14	Q6PJF5	RHBDF2	Inactive rhomboid protein 2	0.0121	0.3839	TNF-α signaling
15	Q9Y2B0	CNPY2	Protein canopy homolog 2	0.0121	0.3839	Cholesterol homeostasis
16	Q9Y4L1	HYOU1	Hypoxia upregulated protein 1	0.1393	0.3839	Cytoprotective role
17	Q15436	SC23A	Protein transport protein Sec23A	0.0121	0.3839	Transportation of proteins from ER to golgi
18	O75844	FACE1	CAAX prenyl protease 1 homolog	0.0121	0.3839	Maintaining normal cell functions

Up- or downregulated proteins were selected on the basis of a cut-off of at least a 10-fold difference in the level as compared to that in uninfected cells. ND indicates protein not detected.