Table of Contents
International Journal of Proteomics
Volume 2015, Article ID 536537, 12 pages
http://dx.doi.org/10.1155/2015/536537
Research Article

A Proteomic Characterization of Bordetella pertussis Clinical Isolates Associated with a California State Pertussis Outbreak

1Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA
2Division of Bacterial Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
3Oak Ridge Institute for Science and Education, Oak Ridge, TN 37831-0117, USA

Received 6 March 2015; Accepted 1 May 2015

Academic Editor: Michael Hippler

Copyright © 2015 Yulanda M. Williamson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table S1 represents a list of the total whole cellular proteome (acid-soluble protein profile) identified among Bordetella pertussis (Bp) Tohama I (T) and the Bp CA2010 outbreak isolates (Bp 2, 4, 5, 9, 11 and 12). Proteins were identified using nano-liquid chromatography-tandem mass spectrometry (nLC-MSMS), database searching and validated using Scaffold (Proteome Software). Information such as Gi accession number, molecular weight, subcellular localization, gene function, gene name, amino acid coverage and number of unique peptides are provided in the table. Table S2 represents a list of the total immunoproteome (a known and putative antigenic protein profile) identified among Bp T and the Bp CA2010 outbreak isolates (Bp 2, 4, 5, 9, 11 and 12). The proteins were identified using antibody affinity capture technologies in parallel with nLC-MSMS, database searching and Scaffold validation. As in the case for table S1, relevant information pertaining to the protein is provided. Figure S1 is a schematic summation of subcellular localization (a) and gene ontology (b) associated with the proteins identified in the whole cellular proteome. Figure S2 is a schematic summation of subcellular localization (a) and gene ontology (b) associated with the proteins identified in the immunoproteome. Lastly, a detailed legend including abbreviations and references cited are provided for each supplementary table or figure.

  1. Supplementary Material