International Journal of Proteomics

Research Article

Miniaturized Digestion and Extraction of Surface Proteins from Candida albicans following Treatment with Histatin 5 for Mass Spectrometry Analysis

Table 1

Proteins identified from a miniaturized assay of HTN5 treatment of C. albicans with decreased MS signals. Average MS signals and their standard deviations were calculated based on six replicates. Proteins indicated with an asterisk () have been determined using their associated protein families. Lower case letters in amino acid sequences indicated modified residues. Blank spaces indicated proteins unidentified by the databases.


Biological function	Observed precursor m/z	Theoretical precursor m/z	MS signal intensities		values	Amino acid sequence	Protein name (UniProt Accession Number)	Charges
Biological function	Observed precursor m/z	Theoretical precursor m/z	Control	HTN5 treated	values	Amino acid sequence	Protein name (UniProt Accession Number)	Charges

Virulence	1284.83	1286.65	27000 ± 9500	14000 ± 2800	0.016	K.sLYTISPNKGK.R	Transcriptional regulator STP3 (A0A0A6LWB8)	3	2.52
	1284.83	1283.69	27000 ± 9500	14000 ± 2800	0.016	K.SKYNLPFAMK.E	Candidapepsin-7 (A0A0A4B714)	3	3.75
	1295.87	1295.70	66000 ± 1100	19000 ± 7000	0.000013	R.tKKWGLGWIK.N	Integrase family (A0A0A3BML7)	1	1.61
	3579.79	3581.47	40000 ± 5000	31000 ± 36000	0.30	HPQYsEACsAVmVVTYSSGSGEHIH TTDIK	Kexin (A0A0A6IYX0)	3	3.33
	3895.36	3892.80	36000 ± 4100	26000 ± 3100	0.0037	ELKTTVIVTSCFNNVCSETsITT PKtAVtATtSK	Flocculin (A0A0A3CM71)	3	3.10
	4659.96	4658.22	15000 ± 1100	3200 ± 2700	0.00014	K.HLTLKSSTPASTLEYSTSIPPALATT SSSLStESTtLttISR.S	Flocculin (A0A0A3CM71)	3	3.25
	4115.51	4115.94	51000 ± 12000	24000 ± 17000	0.017	VTFVEKAtSTSTTNtTttTTTTTTTTTT TTTIPVKR	β-Lactamase family (A0A0A6K6B3)	3	3.23
	4121.03	4122.05	31000 ± 23000	17000 ± 7200	0.13	K.NVKVITTTTTtSPSSFSSSSSLMsPITPQT PNIPKTPK.T	PX domain (A0A0A3CBE8)	3	3.94

Surface-associated	1175.88	1173.66	64000 ± 3900	22000 ± 1100	<0.00001	R.tINLDsQVK.Y	3′(2′),5′-Bisphosphate nucleotidase 2 (A0A0A6NTV1)	1	3.16
Surface-associated	4659.96	4659.87	66000 ± 1100	20000 ± 7000	0.00001	mQTSISttTIEDHLHHYsPEESQKLLSRE SSINTDLFK.E	Bud site selection protein BUD4 (A0A0A3DIG6)	3	3.13

Mitochondrial-associated	1175.88	1175.15	64000 ± 3900	22000 ± 1100	<0.00001	K.YMLLTLLtK.L	YAP-bd/ALF4/glomulin family (A0A0A3CAC1)	1	2.96
	1295.87	1297.68	66000 ± 1100	19000 ± 7000	0.00001	K.LTtLISSIENK.I	YAP-bd/ALF4/glomulin family (A0A0A3CAC1)	1	2.96
	1374.01	1376.70	16000 ± 1300	10000 ± 3000	0.0047	R.TASGNIIPSSTGAAK.A	Glyceraldehyde-3-phosphate dehydrogenase (Q92211)	1	2.86
	4659.96	4660.23	15000 ± 1100	3200 ± 2700	0.00014	K.StIVEEIYsNARSHLVQGNKEmGmALFNE LLAINESIYGK.V	Clustered mitochondria protein homolog (A0A0A4C6G1)	3	3.37

DNA binding	804.77	804.44	42000 ± 22000	19000 ± 3100	0.034	K.QPAVFSR.I	DNA polymerase (Q5A4E5)	1	2.56
	1284.83	1283.53	27000 ± 9500	14000 ± 2800	0.016	R.VASHsLsTsR.R	Minichromosome maintenance protein 10 (A0A0A3CIN8)	1	2.67
	3331.28	3331.42	63000 ± 1200	14000 ± 1400	<0.00001	K.GFTNTMISHIGFDPTGtsLNQNsTS LGsK.S	Minichromosome maintenance protein 10 (A0A0A3CIN8)	3	3.21
	2806.76	2806.20	47000 ± 36000	30000 ± 23000	0.21	R.TTPPTVsITGPNPsSSPAsASTNtSK.S	Phosphatase family (A0A0A3BQA3)	3	3.60
	3376.60	3376.98	43000 ± 12000	32000 ± 28000	0.23	K.SSPTSsATTTATtSVsISSLSLtMGKPKNSK.L	Mediator complex (A0A0A3CP70)	3	3.15
	3498.15	3500.80	34000 ± 8900	18000 ± 16000	0.054	K.LGSVLTtRSQLIEYELtTRtIFINCSA ALK.I	Spt6 (D3IZV2)	3	3.11
	3503.40	3504.74	43000 ± 8300	31000 ± 2500	0.014	R.EVIIKLSITRtHtPPPDSTtTTTPTTSIEK.T	Zn finger domain (A0A0A6J6A5)	3	3.57
	3939.14	3940.46	36000 ± 4000	17000 ± 8000	0.0026	VSSYILDGNNSTKLPsPVLtHtTF DSRsDEGQR	Basic leucine zipper domain (A0A0A3DLJ5)	3	3.99
	4115.51	4116.95	51000 ± 12000	24000 ± 17000	0.017	R.APQsIQLPPIQsFtKsQAVFPQSVRDSAPA ANFNR.Y	Transcription factor and DNA binding protein families (A0A0A6ITE8)	3	3.12
	4659.96	4659.15	15000 ± 1100	3200 ± 2700	0.00014	MKImmIPTHHQtYNINTHQPPQQHQYLPPPGt SYTSPR.A		3	3.12
	4115.51	4114.87	51000 ± 12000	24000 ± 17000	0.017	K.sStQMSSCtNsVTQTLDRLPKIVSTQQNNL TPTSK.I	Zn(2)-DNA binding domain (A0A0A6KY81)	3	3.03
	4659.96	4660.19	15000 ± 1100	3200 ± 2700	0.00014	R.RSVSYSPGPsSIKSQLPHLTSSSTTtssVQSPP PPPPSQPPR.G	Zn(2)-DNA binding domain (A0A0A6KY81)	3	3.61
	4173.60	4173.73	24000 ± 1200	13000 ± 1200	0.000014	K.tVTsINGSPPPLEtAPsSHHNVPIDFIHFK KESDR.T	Uncharacterized protein (A0A0A3EPD4)	3	3.12
	4368.59	4367.89	33000 ± 21000	7100 ± 1300	0.020	K.NMQFPPYQVSsHNsSEtSQsIPNTPSITRQVE SNTR.S	Transcriptional regulatory protein LEU3 (A0A0A3BTY8)	3	3.54
	4368.59	4367.90	33000 ± 21000	7100 ± 1300	0.020	K.tPTTTTTTTTtANGNTSNGNTSNGNsTGKTATA ATATKSNtK	Transcription protein family (A0A0A3C3Y1)	3	3.19

Protein synthesis	804.77	805.41	42000 ± 22000	19000 ± 3100	0.034	K.QTSLNDK.C	Mannosyltransferase family (A0A0A3CUB4)	1	2.54
	804.77	804.45	42000 ± 22000	19000 ± 3100	0.034	R.VKIDSDK.S	Protein transporter SEC24 (A0A0A3EHG4)	1	1.51
	1175.88	1173.98	64000 ± 3900	22000 ± 1100	<0.00001	K.TKQFNDsKK.K	Vesicle tethering protein family (A0A0A6KIG6)	1	2.01
	1284.83	1283.72	27000 ± 9500	14000 ± 2800	0.016	K.tAGsNHNsEK.K	Ribosomal RNA-processing protein 12 (A0A0A4BGJ7)	1	2.51
	4121.03	4119.93	31000 ± 23000	17000 ± 7200	0.13	K.FFsDNIANDLAtTTTTTTTTNTGAtSVHPIL QVDAIK.Y	CAS/CSE protein family (A0A0A6L8P2)	3	3.24
	4368.59	4370.93	33000 ± 21000	7100 ± 1300	0.020	K.ESSSTADQPSVVPPQESHKDTVETPK PEVtEtsVEAtK.E	Translation initiation factor 4G (A0A0A4CDK4)	3	2.99
	4368.59	4367.03	33000 ± 21000	7100 ± 1300	0.020	K.NPTPTPTPTPTPTPNNLAQGVDsSSTLDV EtTLtGLtRR.I	Imidazoleglycerol phosphate synthase cyclase subunit (A0A0A6I238)	3	3.21

Signalling	3503.40	3504.60	43000 ± 8300	31000 ± 2500	0.013	K.RSSITtPtPPLTTTHSSNGNGNGNV NVNVNsK.R	Peroxisome transmembrane receptor (A0A0A3ZWC2)	3	3.30
	4115.51	4116.76	31000 ± 20000	17000 ± 7200	0.017	K.QSTTNTsTLssTtAASTLATSNNTQPD TYTSTSTSIR.G	Pleckstrin homology domain (A0A0A6L5L9)	3	3.33
	4416.51	4414.87	29000 ± 27000	16000 ± 1100	0.10	R.QHPDPLSNQsNFNsNTINNYSNYRS sTRSGLDPsQR.H	Rho GTPase activating protein domain (A0A0A3BMU5)	3	4.12
	4659.96	4660.32	15000 ± 1100	3200 ± 2700	0.00014	K.DLPIGYILHmINLcPNIVsLNLGNLSLsTD YEISRSTIHK.Y	F-box protein COS111 (A0A0A3CYY6)	3	3.68
	4659.96	4658.21	15000 ± 1100	3200 ± 2700	0.00014	K.WNKEKIELDsPLIVSYVSSLCNGGGGGIIT NsTNSTttNSK	Pentatricopeptide repeat (PPR) protein family (A0A0A3CGE7)	3	1.11

Miscellaneous	1284.83	1285.42	27000 ± 9500	14000 ± 2800	0.016	R.NDsDtsLsK.E	Insulin induced protein family (A0A0A3CRM0)	1	1.66
	1284.83	1285.42	27000 ± 9500	14000 ± 2800	0.016	R.YtmNEVFK.V	GYF domain (A0A0A3C0N8)	1	1.66
	3331.28	3331.59	63000 ± 1200	14000 ± 1400	<0.00001	ETsQFMGNAESEDLtGNGLLTSTL AVLSSIS	Orf in C. albicans major repeat sequence, RB2 region (Q5A475)	3	3.15
	3331.28	3332.44	63000 ± 1200	14000 ± 1400	0.23	K.LSSLGNHGTtTTSSLSSSsSsSIsNNT SIAK.I	CBS domain (A0A0A6JTB4)	3	3.46
	3376.60	3376.59	43000 ± 12000	33000 ± 28000	0.23	R.KQQDQNEVAGAAAATTTTAtAtAT AATNWKPK.N	CBS domain (A0A0A6JTB4)	3	3.21
	3498.15	3498.57	34000 ± 8900	18000 ± 16000	0.054	K.SSsLIKNsTsSNQSsPATSTNTSIVDVPIEK.S	WD repeat protein (A0A0A3CA48)	3	3.21
	4173.60	4172.95	24000 ± 1200	13000 ± 1200	0.000014	R.INNNNDKssILsNITTTNTTTGTGTTNTtT VPSIKTK.R	(A0A0A6IY53)	3	4.24
	3498.15	3499.48	34000 ± 8900	18000 ± 16000	0.054	KAFSSsKLTSDSANStNStNsTSMSILGNDK.D	CDK inhibitor PHO81 (A0A0A6MY31)	3	3.16
	3895.36	3894.86	36000 ± 4100	26000 ± 3100	0.0037	R.ISRPNGVGGISTSGSSSPTTEFVTPQAs KsSVDQNKK.R	Ubiquitin interacting motif (C4YNW3)	3	3.29
	4421.03	4421.09	32000 ± 19000	17000 ± 7200	0.080	R.HLILGYKItVVtDHQSLTsVMTSSSRPE NNRMIR.W	Aspartic peptidase family (A0A0A4CVF8)	3	3.83
	3939.14	3940.16	36000 ± 24000	17000 ± 18000	0.0026	R.NVNGSGStNtNTMtRLDsTTIASSLFCRQLYF NLLSK.D	Globin family (A0A0A4CD75)	3	3.11
	4372.57	4373.90	9600 ± 4600	5400 ± 1800	0.058	R.NNsTVSSTNSLmSNNsDTNtAATAATAATSGS TTNNVKR.M	(A0A0A3XE46)	3	3.38
	4416.15	4414.82	29000 ± 27000	16000 ± 1100	0.10	R.NGtYSSsStSSSsTSSVSSSSATTANGESLNST THNIQLER.T	Sec2p (A0A0A6JGN5)	3	2.89
	4659.96	4661.33	15000 ± 1100	3200 ± 2700	0.00014	K.QNYVDPGQIAIKGLKGFVNLGAtCFMssILQtLIHN PLI.K.Y	Ubiquitin carboxyl-terminal hydrolase (C4YKS7)	3	3.69
	4659.96	4660.93	15000 ± 1100	3200 ± 2700	0.00014	K.YDKPmEDTEEIDDVtSISKsINEQID DPFsQFNSVTLR.Y	Protein-serine/threonine kinase (A0A0A4ANB2)	3	3.51

Uncharacterized	1175.88	1173.73	64000 ± 4000	22000 ± 1100	<0.00001	K.tImtItIK.I	(Q59T39)	1	2.07
	3331.28	3332.60	63000 ± 1200	14000 ± 1400	<0.00001	R.IsNIITLNSSLSSSsSsSSSsSSLLLL TLK.S	(Q5AAP9)	3	3.11
	3895.36	3893.70	36000 ± 4100	26000 ± 3100	0.0034	K.TTPVMGNSStPSTVtANtNtGAYSTSSDTA AKPTKK.A	(A0A0A6JKT0)	3	3.14
	3939.14	3940.74	36000 ± 24000	17000 ± 18000	0.0026	K.TTPVMGNSStPSTVtANtNtGAYSTSSDTA AKPTKK.A	(A0A0A6JKT0)	3	3.46
	4115.51	4116.79	31000 ± 20000	17000 ± 7200	0.017	K.TVCNWQtLGHTDEFEEstQFARGVSDtAL VGGITK.L	(Q59K01)	3	2.18
		4114.82				T.CGKSCFINSttANKLIsYNLFQSStEVDS IGPTGSK.T	(Q59KZ4)		3.86
		4116.2				K.TTPVMGNSsTPSTVTANTNTGAYSTSsDt AAKPTKKATK.R	(A0A0A6JKT0)		3.91
		4115.04				R.ILLLLPLLGVtILTSKSSLESISLNsPSDSI ALssSK.S	(Q5AH83)		2.34
	4416.51	4415.18	29000 ± 27000	16000 ± 1100	0.10	K.STSSANIKtKPKPNTATtATTPTAtTATTtA TTSSIDPTEK.D	(A0A0A4CH02)	3	2.59
	4421.03	4419.99	32000 ± 19000	17000 ± 7200	0.080	R.LSGtNNPGsGsGsGGGGGGGANNNSLPGY SVGSSIGRGRGLGR.G	(A0A0A6LWB8)	3	1.89
	4368.59	4366.93	33000 ± 21000	7100 ± 1300	0.020	K.NYNQFKLIELDDSMNTTTTTTTTTTtTttT tTSTIGK.F	(A0A0A6MD82)	3	3.76
	4368.59	4367.87	33000 ± 21000	7100 ± 1300	0.020	K.SKsTTIHNQSNIHSEQISINDENNNKStstS TSTDTK.K	(A0A0A3EQM3)	3	1.32
	4372.57	4370.82	9600 ± 4600	5400 ±1800	0.058	K.SSGGSSDTKSVWIAtTGSDFASQSNSDSs StASRNSSSSASR.Q	(A0A0A3BP13)	3	2.46
	4372.57	4373.17	9600 ± 4600	5400 ±1800	0.058	K.EsEGVWGWsLQCLLLLFTKVNLRscDQL LICALVIR.E	(Q5AKS1)	3	2.99
	4659.96	4661.36	15000 ± 1100	3200 ± 2700	0.00014	K.VILIGNSMTsRTTsSVRFFSIMLTVSLPItN ILVsSELSK.V	(A0A0A3BZ01)	3	3.11