Vital Autofluorescence: Application to the Study of Plant Living Cells

Figure 1

Images and the fluorescence spectra of some intact fluorescing plant secreting cells. Source: adapted from [21, 22] and unpublished data of the review’ author. (a) LSCM images of pollen grains from various species under confocal microscope LSM 510 NLO “Carl Zeiss” (Laser 488 nm). Drops of secretions from apertures are seen. (b) and (c) Views of secretory hairs on leaf of comfrey Symphytum officinale and on the flower calyx of flowering raspberry Rubus odoratus under (A) luminescence microscope Leica 6000 (Excitation 360–380 nm) and (B) laser-scanning confocal microscope Leica TSC SP 5 (laser 405 nm) and the (C) fluorescence spectra measured by registered microspectrofluorimeter MSF-1 (Excitation 360–380 nm) or laser-scanning confocal microscope Leica TSC SP 5 from secretory head with released secretion (1) and stalk (2) of secretory hair.