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International Journal of Spectroscopy
Volume 2013 (2013), Article ID 726820, 8 pages
http://dx.doi.org/10.1155/2013/726820
Research Article

Resolution of Ternary Mixture of Aspirin, Atorvastatin, and Clopidogrel by Chemometric-Assisted UV Spectroscopic and Liquid Chromatography Methods

1Pharmaceutical Analytical Chemistry, Department of Chemistry, Alaqsa University, P.O. Box 4051, Gaza 76888, Palestine
2Analytical Chemistry, Department of Chemistry, Alaqsa University, P.O. Box 4051, Gaza 76888, Palestine
3Inorganic Analytical Chemistry, Department of Chemistry, Alaqsa University, P.O. Box 4051 Gaza 76888, Palestine
4National Institute of Research for Electrochemistry and Condensed Matter, 060021 Bucharest, Romania

Received 26 February 2013; Accepted 20 August 2013

Academic Editor: L. J. Ming

Copyright © 2013 Mahmoud Mohamed Issa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Two chemometrics-assisted UV spectrophotometric methods were proposed for the resolution of ternary mixtures without any chemical pretreatment. The first method is based on modification of H-point standard addition method which permits simultaneous analysis of three species from a unique calibration set by making the simultaneous addition of the three analytes. Quotient between the spectra of aspirin, atorvastatin, and clopidogrel was obtained and the results showed that simultaneous determination of aspirin, atorvastatin, and clopidogrel can be obeyed in the linear range 2.5–20 μg mL−1 of aspirin, 2.5–17.5 μg mL−1 of atorvastatin, and 2.5–20 μg mL−1 of clopidogrel in ternary mixture. The second method is based on the combination of the first derivative spectra and Cramer's matrix rule. In the matrix calculation, clopidogrel has zero crossing point at 316.8 and 212 nm, while for atorvastatin the zero crossing point at 250 nm where the matrix is greatly simplified and easily solved. The linear concentration ranges were 2.5–20 μg mL−1 aspirin, 2.5–17.5 μg mL−1 atorvastatin and 2.5–20 μg mL−1 clopidogrel in ternary mixtures. The results proved that the simultaneous determination of aspirin, atorvastatin, and clopidogrel could be obeyed. Both methods were applied for capsules containing the three ingredients and results were in good concordance with alternative liquid chromatography.