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International Journal of Spectroscopy
Volume 2014 (2014), Article ID 785914, 5 pages
Research Article

FTIR Spectroscopy Combined with Partial Least Square for Analysis of Red Fruit Oil in Ternary Mixture System

Laboratory of Analytical Chemistry, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta 55281, Indonesia

Received 26 February 2014; Accepted 8 May 2014; Published 21 May 2014

Academic Editor: Kam-Sing Wong

Copyright © 2014 A. Rohman et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


FTIR spectroscopy is a promising method for quantification of edible oils. Three edible oils, namely, red fruit oil (RFO), corn oil (CO), and soybean oil (SO), in ternary mixture system were quantitatively analyzed using FTIR spectroscopy in combination with partial least square (PLS). FTIR spectra of edible oils in ternary mixture were subjected to several treatments including normal spectra and their derivative. Using PLS calibration, the first derivative FTIR spectra can be exploited for determination of RFO; meanwhile, the second derivative spectra were preferred for determination of CO and SO. The values obtained for the relationship between actual and FTIR predicted values of RFO, CO, and SO were 0.9863, 0.9276, and 0.9693, respectively. The root mean square error of calibration (RMSEC) values obtained were 1.59, 1.72, and 1.60% (v/v) for RFO, CO, and SO, respectively. The result showed that FTIR spectroscopy offers accurate and reliable technique for quantitative analysis of RFO, CO, and SO simultaneously in ternary mixture systems. Besides, the developed method can be extended for analysis of CO and SO as adulterants in RFO.