Interaction of Small Zinc Complexes with Globular Proteins and Free Tryptophan

Table 1

Percent decrease in fluorescence intensity of fluorophore (1.0 × 10⁻⁵ M) at the highest quencher concentration (21 μM) at 25°C. Fluorescence intensity was measured at 345 nm.

	3	4	5	6
BSA	21.8	—	29.6	4.29
HSA	19.6	16.0	34.6	—
Lyso	23.7	15.2	11.0	—
Trp	—	39.1	—	—
Charge	0	0	0	0

Decrease at 1.8 × 10⁻⁵ M. Quenching reversed at quencher concentrations higher than 1.8 × 10⁻⁵ M.