Table 3: Summary of binding constant, , binding site number, , and for BSA, HSA, lysozyme, and free tryptophan at 25°C.

ComplexBSAHSALysozymeFree Trp
/M−1/kJ mol−1/M−1/kJ mol−1/M−1/kJ mol−1/M−1/kJ mol−1

31.2 × 1051.0−297.7 × 10102.5−566.2 × 1041.0−27
42.0 × 1062.5−663.7 × 1041.0−261.2 × 1051.0−29
51.1 × 1061.0−355.0 × 1061.5−381.7 × 1072.0−41
69.6 × 1041.0−28