Nile Red and 2-NBDG Are Incompatible for the Simultaneous Detection of Lipid and Glucose Accumulation

Figure 2

Fluorescence of 2-NBDG and Nile red in 3T3-L1 adipocytes. (a) Fluorescence micrographs of unstained cells and cells stained with only 2-NBDG, only Nile red, or both simultaneously (costain) excited at 480/40 nm or 560/40 nm and detected with emissions at 527/30 nm or 645/75 nm, respectively. (b) Representative plot profiles of the cells indicated with yellow arrowheads in (a) in the first panel showing the intensities across a single cell when emissions were collected at 527/30 nm and 645/75 nm. Representative images from experiments.

(a)

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