Nile Red and 2-NBDG Are Incompatible for the Simultaneous Detection of Lipid and Glucose Accumulation

Figure 3

Comparison of the excitation and emission spectra of 2-NBDG and Nile red singly stained and costained 3T3-L1 adipocytes. Excitation spectra of samples with emissions detected at (a) 520 nm, (b) 580 nm, and (c) 650 nm. Emission spectra of samples when excited at (d) 480 nm, (e) 520 nm, and (f) 570 nm. Insets show the indicated region enlarged to highlight regions of potential selectivity when comparing single stained to costained adipocytes. The fluorescence intensity is shown as relative to the global maximum for each panel. Representative spectra from experiments.

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