Nile Red and 2-NBDG Are Incompatible for the Simultaneous Detection of Lipid and Glucose Accumulation

Figure 4

Quantification and statistical analysis of the fluorescence of cell stained with only 2-NBDG or Nile red compared to costained cells. (a) Fluorescent intensity with excitation and emission wavelengths set at 480 nm and 510 nm, respectively, (b) 480 nm and 520 nm, respectively, and (c) 520 nm and 580 nm, respectively. Statistical significance was determined with a one-way ANOVA and Tukey’s post hoc test. Different lower case letters indicate significant difference at . The actual signal detected in the costained cells and the expected intensity (determined by summing the singly stained cells) were compared by Student’s t-test. The values represent means ± SD, , ; .

(a)

(b)

(c)