Application of Autofluorescence for Analysis of Medicinal Plants

Figure 4

The fluorescence of terpene-containing secretory cells of hairs from Matricaria chamomilla L. Leaf. ((a) and (b)) Images of the hair in three channels of laser-scanning confocal microscope Leica TCS SP-5 (laser excitation 405 nm) and (c) longitudinal hair optical slices. Flower: (d) image of pollens from flower made by laser-scanning confocal microscope Karl-Zeiss (laser excitation 488 nm); (e) image of the part from ligulate white flower with pollen recorded by laser-scanning confocal microscope Leica TCS SP-5 (laser excitation 405 nm) and its ROI marked by rings (1–5), bar = 30 μm; (f) the fluorescence spectra of the marked ROI (1–5).