Annular Beam Shaping in Multiphoton Microscopy to Reduce Out-of-Focus Background

Figure 3

Schematic illustration of the experimental MPM platform. We introduced an SLM in the excitation path to form an annulus at the back aperture of the objective. M: mirror. L: lens. F: filter. SLM: spatial light modulator. BB: beam blocker. DCM: dichroic mirror. PMT: photomultiplier tube. TCSPC: time-correlated single photon counting data acquisition.