Annular Beam Shaping in Multiphoton Microscopy to Reduce Out-of-Focus Background

<div>MPM images from the dermis of an excised unstained normal human skin specimen applying annular and Gaussian beam illumination at different tissue depths (~45 <i>μ</i>m and ~80 <i>μ</i>m) using matching laser powers (~15 mW and 20 mW, resp.) at the back aperture. Scale bar = 50 <i>μ</i>m.</div>

International Journal of Spectroscopy

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Figure 9

Figure 9: Annular Beam Shaping in Multiphoton Microscopy to Reduce Out-of-Focus Background 