Table of Contents
Influenza Research and Treatment
Volume 2013 (2013), Article ID 360675, 6 pages
http://dx.doi.org/10.1155/2013/360675
Research Article

Monoclonal Antibody Targeting Neutralizing Epitope on H5N1 Influenza Virus of Clade 1 and 0 for Specific H5 Quantification

1Animal Health Biotechnology, Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604
2Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore 117597

Received 8 January 2013; Accepted 4 February 2013

Academic Editor: Prasert Auewarakul

Copyright © 2013 Fang He and Jimmy Kwang. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

H5N1 influenza viruses cause high mortality in avian and mammalian species, including humans. Antigenic drift in H5 sequence poses challenges in the development of vaccine and therapeutic antibody. In this study, a monoclonal antibody 11G12 was produced from inactivated H5N1 immunized mice. Results from IFA, ELISA, HI, and virus neutralization indicated that Mab 11G12 can specifically recognize and neutralize H5 type hemagglutinin from clade 1 and 0 without any cross-reaction to any other clades of H5N1 viruses. Mab 11G12 was used to differentiate and quantify the expression of H5N1 strain A/VietNam/1203/04 from a trivalent vaccine mix in ELISA. Sequencing of escape mutants identified that Mab 11G12 targets a major neutralizing epitope of influenza H5 hemagglutinin. The study indicated that some major neutralizing epitopes in H5s of early strains were mutated due to antigenic drift.