Table of Contents
ISRN Toxicology
Volume 2011 (2011), Article ID 109092, 7 pages
Research Article

Synergistic Cytotoxic Stress and DNA Damage in Clover (Trifolium repens) Exposed to Heavy Metal Soil from Automobile Refining Shops in Kashmir-Himalaya

Department of Botany, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, India

Received 10 November 2011; Accepted 7 December 2011

Academic Editor: K. M. Erikson

Copyright © 2011 Towseef Mohsin Bhat et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Coexposure to heavy metals occurs in many occupational settings, such as automobile refining shops, pigment, and batteries production. Heavy metals around automobile refining shops were tested for their ability to induce synergistic cytogenetic effects in Trifolium repens L. by using the chromosomal aberrasions (CAs), micronucleus (MN) and comet assay. A significant increase in micronucleus (MN), chromosomal abrations (CAs), percentage of nuclei with comet tails (NCTs), the relative comet tail length (CTL), comet tail DNA (CT, DNA), and tail moment (TM) were observed with increased concentration of three heavy metals, like Cd, Pb, Hg. The present result indicate that exposure of T. repens to soils contaminated by heavy metals around automobile refining shops shows clastogenicity, cytotoxicity, and DNA damage at higher concentrations.